Cyclic Guanosine Monophosphate Modulation and Blood Pressure

Cyclic guanosine monophosphate modulation and blood pressure.

Cyclic Guanosine Monophosphate

Since we have previously shown a direct inhibitory effect of platelet-activating factor (PAF) on Cl reabsorption in the medullary thick ascending limb of Henle's loop (TAL), the aim of this study was to extend this effect to the whole TAL and to further investigate the signaling pathway involved. In microperfused cortical TALs, PAF significantly decreased Cl reabsorption by 50.3±6.5%. On the on...

Impaired Nitric Oxide-dependent Cyclic Guanosine Monophosphate

Nitric oxide (NO)-dependent cyclic guanosine monophosphate (cGMP) generation was examined in glomeruli isolated from 1-2-wk and 2-mo streptozotocin diabetic (D) and control (C) rats. After 1-2 wk of diabetes, ex vivo basal cGMP generation and cGMP responses to carbamylcholine (CCh) were significantly suppressed in glomeruli from D compared with those from C, whereas cGMP responses to the calciu...

Radioimmunoassay for adenosine 3', 5'-cyclic monophosphate and guanosine 3',5'-cyclic monophosphate in human blood, urine, and cerebrospinal fluid.

I describe a highly sensitive and very specific assay for cyclic AMP and cyclic GMP in body fluids. An adaptation of the method of Harper and Brooker [j. cyclic Nucleotide Res. 1,207 (1975)], its advantage lies in the fact that the simple acetylation of the samples--a procedure developed by these workers--greatly increases specificity and sensitivity beyond those of previous methods and permits...

Effect of atrial natriuretic peptide on blood pressure, guanosine 3':5'-cyclic monophosphate release and blood volume in uraemic patients.

1. Eleven patients on chronic maintenance dialysis were investigated before and after intravenous bolus injection of atrial natriuretic peptide (2 micrograms/kg body weight). 2. Mean blood pressure was reduced to the same extent in the uraemic patients as in 11 healthy subjects, with a nadir 3 min after the atrial natriuretic peptide injection at which time mean blood pressure was reduced by 13...